Microbiology Products - Consumables

Ethidium Monoazide (EMA) - 50 Monodose microtubes

Ref. 4900015000

Ethidium Monoazide (EMA) mono doses microtubes for individual sample treatment.

In the detection and enumeration of microorganisms in clinical samples, environmental or food control by molecular biology techniques, it is crucial to differentiate live cells from dead cells.

The assessment and understanding of microbial reality may have large biases and alter our conclusions.

The use of reagents will turn the applicability of genetic techniques and applications in many fields, including:

- Clinical Microbiology and infectious disease evolution.
- Pathogen Detection and health risk assessment of environmental and food samples.
- Susceptibility studies: biocides, toxic, antibiotics.
- Microbial ecology.

In this new era, the resolution of molecular techniques can be improved with the living cells differentiation.

Wine vPCR kit for 75 samples

Ref. 4900020000

This kit has been developed to improve the detection of spoilage microorganism during the wine production by viability PCR. This product ensures the optimal sample preparation and the maximum signal PCR reduction of dead cells in wine spoilage microorganisms (bacteria and yeasts), such as Brettanomyces spp.

Ethidium Monoazide (EMA) - 5mg ( 0,5 mg x 10 vials )

Ref. 4900016000

Ethidium Monoazide (EMA) mono doses microtubes for individual sample treatment.

In the detection and enumeration of microorganisms in clinical samples, environmental or food control by molecular biology techniques, it is crucial to differentiate live cells from dead cells.

The assessment and understanding of microbial reality may have large biases and alter our conclusions.

The use of reagents will turn the applicability of genetic techniques and applications in many fields, including:

- Clinical Microbiology and infectious disease evolution.
- Pathogen Detection and health risk assessment of environmental and food samples.
- Susceptibility studies: biocides, toxic, antibiotics.
- Microbial ecology.

In this new era, the resolution of molecular techniques can be improved with the living cells differentiation.

Reaction buffers for photo reactive dyes

Reaction buffers to use in combination with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)

One of the critical steps in all viable cell detection workflows by means of PMA or EMA photoreactions, is sample and reagent incubation in darkness. During this treatment it is very important to maximize reagent diffusion trough dead cells, spores, and cysts of microbial aggregates.

On the other hand it is also necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance needs to be under control. GenIUL introduces three different reaction buffers: Standard, Standard Plus and Anaerobic.

These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.

Reaction Tubes

Ref. 4900019000

The optimal performance of photochemical reactions is highly related with the amount of light received by the sample. In this product the optical properties of plastics and the tube alignment through the PhAST platform have been considered in order to obtain the best- adapted solution.

High transparent 1, 7 mL snap cap micro- centrifuge tubes.

Optimal performance for PhAST Blue platforms.

GenIUL reaction tubes show the best light transmittance at 460 nm (wavelength used for Propidium monoazide or Ethidium monoazide photo-activation). Compared with other suppliers these reaction tubes can obtain up to twofold better transmittance results.

D-Bag (4 units)

Ref. 4900009500

For the removal of DNA intercalating dyes from aqueous solutions.

Nowadays, specific intercalating dyes are widely used for the staining of DNA from electrophoresis to viability PCR. The reagents of Phenanthridinium group as Ethidium Bromide, Propidium Iodide or their derivatives PMA and EMA are mostly used.

Even if the products have theoretical low mutagenic potential, their release to the environment should be avoided in all cases, because they can be toxic and/or mutagenic. The D-Bag system is a safe and effective solution for the removal of DNA dyes in aqueous solution, which minimizes waste storing.

PEMAX™ - Reagent 2 vials of 0,5 mg

Ref. 4900013000

PEMAX™ a double dye technology (Patent pending) developed by GenIUL in order to overcome the current limitations of viability PCR procedures. Nowadays, the paradigm is based on cell membrane integrity of living cells as a unique differential factor from dead cells. However, as appointed in first reports, some treatment procedures can induce cell deaths without compromising membrane integrity.

This new reagent, combined with the appropriate reaction buffer, extends the concept of viability PCR to cells with intact cell membrane structure but also with the capability to actively maintain bacterial homeostasis, as a result of active metabolism.

PEMAX™ Reagent 25 monodoses (TBC – Biomarker Kit)

Ref. 4900013000

Reaction buffers to use in combination with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)

One of the critical steps in all viable cell detection workflows by means of PMA or EMA photoreactions, is sample and reagent incubation in darkness. During this treatment it is very important to maximize reagent diffusion trough dead cells, spores, and cysts of microbial aggregates.

On the other hand it is also necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance needs to be under control. GenIUL introduces three different reaction buffers: Standard, Standard Plus and Anaerobic.

These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.

PEMAX™ Reagent 50 monodoses (Std. Buffer)

Ref. 4900013150

This kit has been developed as a universally ready to use solution for vPCR. It can easily be used in common applications such as pathogen detection in water. i.e Legionella.

Legionella spp. qPCR detection Kit for 50 reactions

Ref. 4900023000

Designed and validated for vPCR
Very stable reaction mix, not need ice during transport or during PCR setup. Includes DNA standard.
Includes qualitative internal PCR control.
Large PCR amplicon for ensuring the best vPCR resolution.
Multiple Intercalating Dye Technology (MIDtech), for ensuring a better DNA binding profile in large amplicons.